Profile

Verena Siewers, PhD

Department of Biology and Biological Engineering,
Chalmers University of Technology,
Kemivägen 10, SE-412 96 Göteborg, Sweden

Phone: +46(0)31 772 3853
Fax: +46(0)31 772 3801
E-mail: siewers [at] chalmers.se
Office: Room 3055B

 
Education and degrees
2000 Diploma in Biology, University of Münster, Germany
2004 PhD, University of Münster, Germany
Employments
2001-2005 Research Associate, Department of Biology, University of Münster, Germany
2005-2008 PostDoc, Department of Systems Biology, Technical University of Denmark
2008-2010 Researcher, Department of Chemical and Biological Engineering, Chalmers
2010- Project leader, Department of Chemical and Biological Engineering, Chalmers
Publications
2004 Rolke Y, Liu S, Quidde T, Williamson B, Schouten A, Weltring K-M, Siewers V, Tenberge KB, Tudzynski B, Tudzynski P. 2004. Functional analysis of H2O2-generating systems in Botrytis cinerea: the major Cu-Zn-superoxide dismutase (BCSOD1) contributes to virulence on French bean, whereas a glucose oxidase (BCGOD1) is dispensable. Mol Plant Pathol 5:17-23.
  Siewers V, Smedsgaard J, Tudzynski P. 2004. The P450 monooxygenase BcABA1 is essential for abscisic acid biosynthesis in Botrytis cinerea. Appl Environ Microbiol 70:3868-3876.
  Tudzynski P, Siewers V. 2004. Approaches to molecular genetics and genomics of Botrytis. In: Y Elad, B Williamson, P Tudzynski, N Delen (eds.). Botrytis: biology, pathology and control. Kluwer Academic Press, pp 53-66.
   
2005 Siewers V, Viaud M, Jimenez-Teja D, Collado IG, Schulze Gronover C, Pradier J-M, Tudzynski B, Tudzynski P. 2005. Functional analysis of the cytochrome P450 monooxygenase gene bcbot1 of Botrytis cinerea indicates that botrydial is a strain-specific virulence factor. Mol Plant-Microbe Interact 18:602-612.
  Chagué V, Danit L-V, Siewers V, Schulze Gronover C, Tudzynski P, Tudzynski B, Sharon A. 2005. Ethylene sensing and gene activation in Botrytis cinerea: the missing link in ethylene regulation of fungal – plant interactions? Mol Plant-Microbe Interact 19:33-42.
   
2006 Siewers V, Kokkelink L, Smedsgaard J, Tudzynski P. 2006. Identification of an abscisic acid gene cluster in the grey mould Botrytis cinerea. Appl Environ Microbiol 72:4619-4626.
   
2009 Flagfeldt DB, Siewers V, Huang L, Nielsen J. 2009. Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae. Yeast 26:545-551.
  Siewers V, Chen X, Huang L, Zhang J, Nielsen J. 2009. Heterologous production of non-ribosomal peptide LLD-ACV in Saccharomyces cerevisiae. Metab Eng 11:391-397.
  Siewers V, Mortensen, U, Nielsen J. 2009. Genetic engineering tools for Saccharomyces cerevisiae. In: AL Demain, R Baltz, JE Davies (eds.). Manual of industrial microbiology and biotechnology, 3rd edition, ASM press, pp 287-301.
   
2010 Siewers V, San-Bento R, Nielsen J. 2010. Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisiae. Biotech Bioeng 106:841-844.
  Partow S, Siewers V, Bjørn S, Nielsen J, Maury J. 2010. Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae. Yeast 27:955-964.
  Papini M, Nookaew I, Scalcinati G, Siewers V, Nielsen J. 2010. Phosphoglycerate mutase knock-out mutant Saccharomyces cerevisiae: physiological investigation and transcriptome analysis. Biotechnol J 5:1016-1027.
   
2011 Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J. 2011. Prospects for microbial biodiesel production. Biotechnol J 6:277-285.
  Krivoruchko A, Siewers V, Nielsen J. 2011. Opportunities for yeast metabolic engineering: Lessons from synthetic biology. Biotechnol J 6:262-276.
  Van Mulders SE, Stassen C, Daenen L, Devreese B, Siewers V, van Eijsden RG, Nielsen J, Delvaux FR, Willaert R. 2011. The influence of microgravity on invasive growth in Saccharomyces cerevisiae. Astrobiology 11:45-55.
   
2012 de Jong B, Siewers V, Nielsen J. 2012. Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels. Curr Opin Biotechnol 23:624-630.
  Kim IK, Roldão A, Siewers V, Nielsen J. 2012. A systems-level approach for metabolic engineering of yeast cell factories. FEMS Yeast Res 12:228-248.
  Scalcinati, G, Knuf C, Partow S, Chen Y, Maury J, Schalk M, Daviet L, Nielsen J, Siewers V. 2012. Dynamic control of gene expression in Saccharomyces cerevisiae engineered for the production of plant sesquiterpene α-santalene in a fed-batch mode. Metab Eng 14:91-103.
  Shi S, Valle-Rodríguez JO, Khoomrung S, Siewers V, Nielsen J. 2012. Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production. Biotechnol Biofuels 5:7.
  Papini M, Nookaew I, Siewers V, Nielsen J. 2012. Physiological characterization of recombinant Saccharomyces cerevisiae expressing the Aspergillus nidulans phosphoketolase pathway: validation of activity through (13)C-based metabolic flux analysis. Appl Microbiol Biotechnol 95:1001-1010.
  Chen Y, Partow S, Scalcinati G, Siewers V, Nielsen J. 2012. Enhancing the copy number of episomal plasmids in Saccharomyces cerevisiae for improved protein production. FEMS Yeast Res 12:598-607.
  Chen Y, Siewers V, Nielsen J. 2012. Profiling of cytosolic and peroxisomal acetyl-CoA metabolism in Saccharomyces cerevisiae. PLoS One 7:e42475.
  Scalcinati G, Partow S, Siewers V, Schalk M, Daviet L, Nielsen J. 2012. Combined metabolic engineering of precursor and co-factor supply to increase alpha-santalene production by Saccharomyces cerevisiae. Microb Cell Fact 11:117.
  Kocharin K, Chen Y, Siewers V, Nielsen J. 2012. Engineering of acetyl-CoA metabolism for the improved production of polyhydroxybutyrate in Saccharomyces cerevisiae. AMB Express 2:52.
  Partow S, Siewers V, Daviet L, Schalk M, Nielsen J. 2012. Reconstruction and evaluation of the synthetic bacterial MEP pathway in Saccharomyces cerevisiae. PLoS One 7:e52498.
   
2013 Chen Y, Daviet L, Schalk M, Siewers V, Nielsen J. 2013. Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab Eng 15:48-54.
  van Eijsden RG, Stassen C, Daenen L, Van Mulders SE, Bapat PM, Siewers V, Goossens KV, Nielsen J, Delvaux FR, Van Hummelen P, Devreese B, Willaert RG. 2013. A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study. Biotechnol Lett 35:891-900.
  Kocharin K, Siewers V, Nielsen J. 2013. Improved polyhydroxybutyrate production by Saccharomyces cerevisiae through the use of the phosphoketolase pathway. Biotechnol Bioeng 110:2216-2224.
  Buijs NA, Siewers V, Nielsen J. 2013. Advanced biofuel production by the yeast Saccharomyces cerevisiae. Curr Opin Chem Biol 17:480-488.
  Krivoruchko A, Serrano-Amatriain C, Chen Y, Siewers V, Nielsen J. 2013. 1. Improving biobutanol production in engineered Saccharomyces cerevisiae by manipulation of acetyl-CoA metabolism. J Ind Microbiol Biotechnol 40:1051-1056.
  Tippmann S, Chen Y, Siewers V, Nielsen J. 2013. From flavors and pharmaceuticals to advanced biofuels: Production of isoprenoids in Saccharomyces cerevisiae. Biotechnol J 8 :1435-1444.
   
2014 Valle-Rodríguez JO, Shi S, Siewers V, Nielsen J. 2014. Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways. Appl Energy 115:226-232.
  Chen Y, Bao J, Kim IK, Siewers V, Nielsen J. 2014. Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae. Metab Eng 22:104-109.
  Siewers V. 2014. An overview on selection marker genes for transformation of Saccharomyces cerevisiae. Methods Mol Biol 1152:3-15.
  de Jong BW, Shi S, Siewers V, Nielsen J. 2014. Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway. Microb Cell Fact 13:39.
  Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J. 2014. Engineering of chromosomal wax ester synthase integrated Saccharomyces cerevisiae mutants for improved biosynthesis of fatty acid ethyl esters. Biotechnol Bioeng 111:1740-1747.
  Shi S, Chen Y, Siewers V, Nielsen J. 2014. Improving production of malonyl coenzyme A-derived metabolites by abolishing Snf1-dependent regulation of Acc1. MBio 5:e01130-14.
 

David F, Siewers V. Advances in yeast genome engineering. FEMS Yeast Res 15:1-14.

  Zhou YJ, Buijs NA, Siewers V, Nielsen J. 2014. Fatty acid-derived biofuels and chemicals production in Saccharomyces cerevisiae. Front Bioeng Biotechnol 2:32.
  Krivoruchko A, Zhang Y, Siewers V, Chen Y, Nielsen J. 2014. Microbial acetyl-CoA metabolism and metabolic engineering. Metab Eng 28C:28-42.
  Chen Y, Zhou YJ, Siewers V, Nielsen J. 2014. Enabling technologies to advance microbial isoprenoid production. Adv Biochem Eng Biotechnol 148:143-160.
  de Jong BW, Shi S, Valle-Rodríguez JO, Siewers V, Nielsen J. 2014. Metabolic pathway engineering for fatty acid ethyl ester production in Saccharomyces cerevisiae using stable chromosomal integration. J Ind Microbiol Biotechnol 42:477-486.
  Navarrete C, Nielsen J, Siewers V. 2014. Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing. AMB Express 4:86.
   
2015 Buijs NA, Zhou YJ, Siewers V, Nielsen J. 2015. Long-chain alkane production by the yeast Saccharomyces cerevisiae. Biotechnol Bioeng 112:1275-1279.
  Chen Y, Zhang Y, Siewers V, Nielsen J. 2015. Ach1 is involved in shuttling mitochondrial acetyl units for cytosolic C2 provision in Saccharomyces cerevisiae lacking pyruvate decarboxylase. FEMS Yeast Res 15:fov015.
  Goossens KV, Ielasi FS, Nookaew I, Stals I, Alonso-Sarduy L, Daenen L, Van Mulders SE, Stassen C, van Eijsden RG, Siewers V, Delvaux FR, Kasas S, Nielsen J, Devreese B, Willaert RG. 2015. Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival. MBio 6:e00427-15.
  Zhang Y, Dai Z, Krivoruchko A, Chen Y, Siewers V, Nielsen J. 2015. Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth. FEMS Yeast Res 15:fov024.
  López J, Essus K, Kim IK, Pereira R, Herzog J, Siewers V, Nielsen J, Agosin E. 2015. Production of β-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae. Microb Cell Fact 14:84.
  Tippmann S, Scalcinati G, Siewers V, Nielsen J. 2015. Production of farnesene and santalene by Saccharomyces cerevisiae using fed-batch cultivations with RQ-controlled feed. Biotechnol Bioeng 113:72-81.
  Zhang Y, Liu G, Engqvist MK, Krivoruchko A, Hallström BM, Chen Y, Siewers V, Nielsen J. 2015. Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain. Microb Cell Fact 14:116.
  Qin J, Zhou YJ, Krivoruchko A, Huang M, Liu L, Khoomrung S, Siewers V, Jiang B, Nielsen J. 2015. Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine. Nat Commun 6:8224.
  Fletcher E, Feizi A, Kim S, Siewers V, Nielsen J. 2015. RNA-seq analysis of Pichia anomala reveals important mechanisms required for survival at low pH. Microb Cell Fact 14:143.
  de Jong BW, Siewers V, Nielsen J. 2015. Physiological and transcriptional characterization of Saccharomyces cerevisiae engineered for production of fatty acid ethyl esters. FEMS Yeast Res 16:fov105.
  Shi S, Ji H, Siewers V, Nielsen J. 2015. Improved production of fatty acids by Saccharomyces cerevisiae through screening a cDNA library from the oleaginous yeast Yarrowia lipolytica. FEMS Yeast Res 16:fov108.
   
2016 David F, Nielsen J, Siewers V. 2016. Flux control at the malonyl-CoA node through hierarchical dynamic pathway regulation in Saccharomyces cerevisiae. ACS Synth Biol (in press)
Personal Text
Verena Siewers is a project leader at the Department of Chemical and Biological Engineering in the Systems and Synthetic Biology Group. Her main research interest is the application of yeast as a cell factory for sustainable production of a variety of chemicals as well as advanced biofuels. This also includes the development of synthetic biology tools to improve the construction of efficient yeast strains. Verena Siewers is furthermore involved in the Novo Nordisk Foundation Center for Biosustainability and the FORMAS Center for Metabolic Engineering.

 

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